New primers for sex identification in the Chukar partridges (Alectoris chukar)

ABSTRACT

1. Male and female Chukar partridges are difficult to differentiate based on their morphology or by the Chromobox-Helicase-DNA binding (CHD) during early growth.

2. The current study developed a novel, simple, low-cost and rapid sexing protocol for Chukar partridges based on the newly defined sexing gene ubiquitin-associated protein 2 (UBAP2).

3. The length of polymorphism between UBAP2-W and UBAP2-Z homologous genes allows for easy sex discrimination in this species. Molecular sexing analysis was based on the simultaneous amplification of both genes, resulting in two distinct amplicons (947 bp and 535 bp) in heterogametic females and only a single band (535 bp) in homogametic males, which is easy to detect with agarose gel electrophoresis.

4. This technique is simple and convenient for genetic sex determination in Chukar partridges.

KEYWORDS:

• Sex identification
• Chukar partridges
• UBAP2
• CHD
• capillary electrophoresis

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This work was supported by a grant from the Natural Science Foundation of Jiangsu Province [BK20221412], the National Key Research and Development Program of China [2021YFD1200302] and “JBGS” Project of Seed Industry Revitalization in Jiangsu Province [No. JBGS〔2021〕029].