Synthesis and application of a novel fluorescent probe based on benzothiazole for detection of hydrazine in real samples

ABSTRACT

A novel fluorescence enhancement type probe 2-(1, 3-benzothiazol-2-yl)-5-(diethylamino)phenyl thiophene-2-carboxylate (PTN) for the detection of hydrazine (N₂H₄) was designed and synthesised. The fluorescence of the probe PTN solution increased dramatically upon the addition of N₂H₄ and its appearance changed from nearly colourless to dazzling blue. The simple structure and readily available fluorescent probe provide a novel approach for the quantitative detection of N₂H₄ in the linear range from 0 to 37.5 μM, with a detection limit down to 56 nM, fast response (within 5 min), strong anti-interference ability, easy operation (test paper), and a wide range of pH from 6 to 10. Furthermore, the probe PTN was able to detect N₂H₄ in actual water samples and image the exogenous N₂H₄ signal in colorimetric test strips and living zebrafish.

KEYWORDS:

• Fluorescent probe
• hydrazine
• synthesis
• benzothiazole
• reaction-based

Acknowledgments

The authors are grateful for financial support from the Fundamental Research Funds for the natural science foundation of Sichuan Nursing Vocational College [No. 2022RZY03].

Disclosure statement

No potential conflict of interest was reported by the author(s).

Supplementary material

Supplemental data for this article can be accessed online at https://doi.org/10.1080/03067319.2024.2351193

Additional information

Funding

The work was supported by the Sichuan Nursing Vocational College [2022RZY03].