Development and efficacy assessment of polyherbal phytosomal gel for accelerated wound healing

Abstract

Curcuma longa L. and Plumbago zeylanica L. are renowned for their antioxidant, anti-inflammatory, and wound-healing properties, primarily attributed to their polyphenolic compounds. However, the limited water solubility of these compounds poses challenges to their effective utilization. Encapsulation within phytosomes offers a solution by enhancing bioavailability and permeability. This study aimed to formulate a phytosome-based polyherbal gel incorporating methanolic extracts of P. zeylanica and C. longa to explore its potential in wound healing. Methanolic extracts of P. zeylanica roots and C. longa rhizomes were encapsulated in phytosomes using the lipid film hydration technique. Various phytosome formulations were developed and characterized for encapsulation efficiency, particle size, polydispersity index and zeta potential. The optimized phytosomal dispersion (F7) was integrated into a carbopol-based hydrogel matrix. In vitro release studies demonstrated prolonged release compared to conventional forms. Stability testing confirmed the robustness of the phytosomal gel at 4 °C/60 ± 5% RH. Wound healing activity was assessed using an excision wound model. The phytosomal gel exhibited enhanced wound contraction and reduced epithelization time compared to conventional gel and control groups, signifying its potent wound-healing effect. In conclusion, the polyherbal phytosomal gel, incorporating P. zeylanica and C. longa, holds promise in promoting wound healing, presenting a novel and effective approach in the realm of topical formulations for wound care.

Keywords:

• C. longa
• P. zeylanica
• curcumin
• plumbagin, phytosomes
• wound healing

Acknowledgments

The authors are thankful to the Faculty of Pharmacy, Integral University, Lucknow and Hygia Institute of Pharmaceutical Education and Research, Lucknow for providing all necessary facilities related to the present work (Manuscript Communication Number: IU/R &D/2021-MCN0001263).

Availability of data and materials

The authors confirm that the data supporting the findings of this study are available within the article.

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Not applicable.

Disclosure statement

The authors declare no conflict of interest, financial or otherwise.

Additional information

Funding

None.