Abstract
Purpose. This study was designed to investigate the presence of the secretory phospholipase A 2 receptor in RPE, a protein closely related to the phagocytic mannose receptor. Methods. Proteins from cultured rat, pig, and human RPE were separated by SDS-PAGE and immunoblotted with a polyclonal guinea pig sPLA 2 receptor antibody. RT-PCR was performed on rat and pig RPE samples using primers designed from published rat pancreatic sPLA 2 receptor sequences. Results. The sPLA 2 receptor protein was not detected in rat, pig, or human RPE by immunoblots. Additionally, message for this receptor was not detected in rat or pig RPE. Conclusions. With these techniques, these data demonstrate that the sPLA 2 receptor is undetectable in the RPE.