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Abstract
Callus and cell suspension cultures were established from the stem explants of the plant Tinospora cordifolia. Accumulation of berberine and jatrorrhizine (protoberberine alkaloids) was observed in both callus and cell suspension cultures. The alkaloids were also exudated into the surrounding nutrient medium by non-immobilized and immobilized cultures. The root extracts of T. cordifolia showed higher levels of jatrorrhizine compared to the levels of berberine. An HPLC method was standardised for the separation, identification and quantification of these alkaloids.