Chromosome 8 Aneuploidy in Acquired Cholesteatoma

Abstract

Objective To investigate the incidence of chromosome 8 aneuploidy in acquired cholesteatoma.

Material and Methods Cholesteatoma tissue and postauricular skin as a control were surgically obtained from 12 patients with acquired cholesteatoma. Fluorescence in situ hybridization (FISH) analysis using a chromosome 8-specific α-satellite DNA probe was performed on the interphase nuclei. Two hundred cells were analyzed for each of the samples.

Results Chromosome 8 aneuploidy was found in 9/12 patients whereas a normal cell structure with 2 signals was observed in the remaining 3 patients. In samples with chromosome 8 aneuploidy, the mean proportion of aneuploidy was 25.6%, including monosomy (3.2%), trisomy (16.1%), tetrasomy (4.9%) and more than tetrasomy (1.4%). The number of aneuploid cells in recurrent cases was more than that in non-recurrent cases.

Conclusion A numerical aberration of chromosome 8 was found in patients with acquired cholesteatoma. Our results support the hypothesis that chromosome 8 may be a prognostic factor for cholesteatoma and an indicator in the follow-up of patients with cholesteatoma.

• Cholesteatoma
• Chromosome 8
• Fluorescence in situ hybridization
• Localization
• Recurrence