Evaluation of the protective effect of Botulinum toxin type A injection on otitis media with effusion^*

Abstract

Objective: This study makes an analysis of the effect of Botulinum toxin type A on otitis media with effusion in rats.

Method: As part of the study, 24 male Wistar Albino rats were divided into three groups: Group 1: Botulinum toxin Type A and Histamıne (intratympanic 0.2 ml = 20 unit BTA); Group 2: Saline and Histamine (intratympanic 0.2 ml 0.9%); Group 3: Histamine (intratympanic 0.2 ml). Histamine (intratympanic 0.2 ml) was administered into the right ear for all groups. DPOAE and ABR tests were carried out on days 5, 10, 15 and 20 from the beginning of the study.

Results: There was no significant difference between DPOAE and ABR scores of the groups. In each group, the DPOAE scores for the right ear significantly decreased on day 5 when compared to the basal scores. In each group, there was no significant difference between days 5, 10 and 15 for the right ear.

Conclusions: Botulinum toxin type A blocked the secretion of glands in the middle ear and showed no effect to prevent the formation of serous otitis. In addition, it was found out that Botulinum toxin did not lead to any morphological change in the cochlea

Chinese abstract

目的: 本研究分析A型肉毒杆菌毒素对大鼠有渗出现象的中耳炎的影响。

方法: 作为研究的一部分, 将24只雄性Wistar 白化大鼠分为3组。第1组: A型肉毒毒素和组胺 (鼓室内0.2ml =20单位BTA) ；第2组: 盐水和组胺 (鼓室内0.2ml 0.9%) ；第3组: 组胺 (鼓室内0.2ml) 。组胺 (鼓室内0.2ml) 施予所有组的右耳。 DPOAE和ABR测试在研究开始的第5、10、15和20天进行。

结果: 每组的DPOAE与ABR评分差异无统计学意义。与基础分相比, 在第5天, 右耳的DPOAE评分显著降低。在每组中, 右耳第5、10和15天之间没有显著差异。

结论: A型肉毒杆菌毒素阻断了中耳分泌腺体, 没有发现它具有防止浆液性耳炎形成的作用。此外, 据发现, 肉毒杆菌毒素不会导致耳蜗内任何形态变化。

Keywords:

• Botulinum toxin
• serous otitis media
• rat
• otitis media
• hearing loss

Disclosure statement

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this article.