Disposable Nanosensor for the Electrochemical Determination of Temozolomide and İts Interaction with Double-Stranded DNA

Abstract

Novel disposable screen-printed electrodes modified with silicon dioxide nanoparticles were developed for the electrochemical determination of temozolomide and its interaction with DNA. The characterization of silicon dioxide nanoparticles (SiNPs) was conducted by transmission electron microscopy and energy-dispersive x-ray spectroscopy (EDX). The EDX measurements show that the silicon nanoparticles consisted of 53.08% oxygen (O) and 46.92% silicon (Si), confirming their purity. Chemical characterization was conducted using Fourier transform infrared spectroscopy. Surface characterization of SiNPs-modified screen-printed electrodes was performed using atomic force microscopy. SiNP modification was demonstrated to increase the electrode surface area by 2.3-folds, from 0.75 × 10⁻³ cm² to 1.75 × 10⁻³ cm², resulting in an enhanced signal. The detection limit was 5.6 µg/mL for temozolomide and 7.2 µg/mL for DNA, respectively. The linear concentration range was from 18.6 to 200 µg/mL for temozolomide and 23.9 to 80 µg/mL for DNA.

Keywords:

• Differential pulse voltammetry
• electrochemistry
• nanosensor
• temozolomide-DNA interaction

Acknowledgments

This research was conducted by the first author as a Doctoral Thesis under the supervision of Prof. Mihrican Muti at the Department of Chemistry, Faculty of Sciences, Aydın Adnan Menderes University. The authors extend their gratitude to the Scientific Research Unit of Aydın Adnan Menderes University for the financial support (Project Number: FEF 20022). F. A. expresses appreciation for the doctoral scholarship awarded by TUBITAK 2211 C and the Ph.D. program of the Higher Education Council of Turkey (YÖK) 100/2000.

Author contributions

Fatma Akpınar: methodology, software, investigation, formal analysis, writing—original draft. Mihrican Muti: conceptualization, methodology, formal analysis, visualization, writing—original draft, writing—review and editing, supervision, project administration.

Disclosure statement

No potential conflict of interest was reported by the authors.