INVOLVEMENT OF P42/44 MAPK IN THE EFFECTS OF ETHANOL ON SECRETION OF INSULIN-LIKE GROWTH FACTOR (IGF)-I AND INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN (IGFBP)-1 IN PRIMARY CULTURED RAT HEPATOCYTES

SUN-MI LEE Department of Physiology, College of Veterinary Medicine, Chonbuk National University, Jeonju, Republic of Korea

RAFIQUL ALAM Department of Physiology, College of Veterinary Medicine, Chonbuk National University, Jeonju, Republic of Korea

CHA JUNG HO Department of Physiology, College of Veterinary Medicine, Chonbuk National University, Jeonju, Republic of Korea

JONG-HOON KIM Department of Physiology, College of Veterinary Medicine, Chonbuk National University, Jeonju, Republic of Korea

CHANG-WON KANG Department of Physiology, College of Veterinary Medicine, Chonbuk National University, Jeonju, Republic of Korea

JAE HONG PARK Department of Animal Resources and Biotechnology, Chonbuk National University, Jeonju, Republic of Korea

MYEONG SOO LEE Center for Integrative Medicine, Institute of Medical Science, Wonkwang University, Iksan Chonbuk, Republic of Korea

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Abstract

This article investigates the effects of ethanol on Insulin-like growth factor (IGF)-I secretion, p42/44 mitogen-activated protein kinase (MAPK) activity, and IGF binding protein (IGFBP-1 secretion) in primary cultured rat hepatocytes. The p42/44 MAPK activity increased with the ethanol concentration compared to control after ethanol treatment. The secretion of IGF-I significantly increased compared to control, but IGFBP-1 secretion was inhibited. Treatment with 4-methylpyrazole blocked the IGF-I and IGFBP-1 secretion and p42/44 MAPK activity. Increased IGF-I secretion and inhibited IGFBP-1 secretion due to ethanol-induced p42/44 MAPK activity (at 30 min) was blocked by treatment with PD98059. Taken together, these results suggest that ethanol is involved in the modulation of the secretion of IGF-I and IGFBP-1 by p42/44 MAPK in primary cultured rat hepatocytes. In addition, inhibition of p42/44 MAPK activity by ethanol occurs via the activity of ADH.

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INVOLVEMENT OF P42/44 MAPK IN THE EFFECTS OF ETHANOL ON SECRETION OF INSULIN-LIKE GROWTH FACTOR (IGF)-I AND INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN (IGFBP)-1 IN PRIMARY CULTURED RAT HEPATOCYTES

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INVOLVEMENT OF P42/44 MAPK IN THE EFFECTS OF ETHANOL ON SECRETION OF INSULIN-LIKE GROWTH FACTOR (IGF)-I AND INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN (IGFBP)-1 IN PRIMARY CULTURED RAT HEPATOCYTES

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