![Sample our Bioscience journals, sign in here to start your access, Latest two full volumes FREE to you for 14 days]({"type":"image" , "src" : "/sda/5925/TOC-Subject-Sample-2021-Bioscience.jpg"})
Abstract
We investigated whether X-ray radiation induced apoptosis of the proliferative ependymal cells (ECs) in adult rats with spinal cord injury (SCI) and the effect of X-ray radiation on the proliferative activities of ECs. A rat model with SCI was developed and used to determine the proliferation and apoptosis of ECs in the spinal cords after X-ray exposure. TUNEL assay and BrdU incorporation were used to detect apoptosis and proliferation respectively. We found that there were few TUNEL-positive cells in proliferative ependymal zone (EZ) after SCI except at the epicenter, and approximately half of the irradiated ECs became TUNEL-positive. However, these radiated ECs did not lose their proliferative activity until 1 week later and started to decrease rapidly after 1 week. The observation suggested that only part of ECs were sensitive to radiation and the nonsensitive cells continued their mitosis process. These findings indicated that X-ray exposure of the rats with SCI in early stage induced apoptosis of the proliferative ECs and partially inhibited their proliferative activities.
| Abbreviations: | ||
| SCI | = | spinal cord injury |
| NSCs/NPCs | = | neural stem/precursor cells |
| ECs | = | ependymal cells |
| TUNEL | = | terminal deoxynucleotidy transferase-mediated dUTP nick end labeling |
| FITC | = | fluorescein isothiocyanate |
| TRITC | = | tetraethyl rhodamine isothiocyanate |
| EZ | = | ependymal zone |
| PBS | = | phosphate buffered saline |
| PB | = | phosphate buffer |
| Abbreviations: | ||
| SCI | = | spinal cord injury |
| NSCs/NPCs | = | neural stem/precursor cells |
| ECs | = | ependymal cells |
| TUNEL | = | terminal deoxynucleotidy transferase-mediated dUTP nick end labeling |
| FITC | = | fluorescein isothiocyanate |
| TRITC | = | tetraethyl rhodamine isothiocyanate |
| EZ | = | ependymal zone |
| PBS | = | phosphate buffered saline |
| PB | = | phosphate buffer |