Phagocyte‐derived oxidants and plasma antioxidants in haemodialysed patients

Abstract

Objective. Oxidative stress is one of the important complications occurring in haemodialysis. The aim of the study was to determine haemodialysis‐induced changes in oxidative burst of phagocytes and the antioxidative properties of plasma. Methods. Twenty‐seven patients and 50 healthy controls were examined. Oxidative burst of phagocytes and plasma antioxidative potential were measured luminometrically. Concentrations of major plasma antioxidants (vitamin E, bilirubin and uric acid) were also determined. Results. Phagocyte chemiluminescence was higher in patients before haemodialysis compared with that in controls and decreased after haemodialysis compared with predialysis status. A significant increase in plasma antioxidative potential and uric acid was found in patients before haemodialysis. These parameters decreased after haemodialysis compared with both predialysis and control values. Conclusions. The higher generation of phagocyte‐derived oxidants and the decline in plasma antioxidative properties after haemodialysis confirm insufficient antioxidant defence in patients with chronic renal failure.

• Chemiluminescence
• hematology
• immunity
• kidney diseases
• markers of oxidative stress

Acknowledgement

This work was supported by the grant no. 4796‐3 (Internal Grant Agency of the Ministry of Health of the Czech Republic) and grant no. 1QS 500040507 AS CR.