Spectrophotometric Determination of Oxygen Saturation in Capillary Blood

Abstract

The oxygen saturation of capillary blood (approximately 25 μpl) is determined spectrophotometrically on hemolyzed packed red cells at 600 and 505 nm. The blood is centrifuged and the packed red cells hemolyzed by successive freezing and thawing. For a 0.1 mm cuvette an ordinary blood counting chamber is found convenient.