Clinical Chemistry: Electrophoretic Separation, Detection, and Variation of Amylase Isoenzymes

Abstract

Skude, G. Electrophoretic Separation, Detection, and Variation of Amylase Isoenzymes. Scand. 1. clin. Lab. Invest. 35, 41–47, 1975.

An electrophoretic technique for demonstrating amylase isoenzymes is described. After separation in an agarose gel containing a linear polyacryl-amide polymer to reduce electroendosmotic flow, the amylase fractions are visualized by incubation with a commercially available dye-starch polymer (PhadebasB Amylase Test). The technique detects amylase fractions with activities below 10 U/l. Some characteristic changes in such diseases as acute and chronic pancreatitis, cystic fibrosis of the pancreas, macroamylasemia and inherited variants as well as after maxillofacial surgery are mentioned.

Key Words:

• Agarose gel electrophoresis
• amylase
• amylase isoenzymes
• cystic fibrosis
• macroamylasemia
• pancreatitis