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Abstract
A procedure for determination of serum alkaline phosphatase activity (EC 3.1, 3.1.) in diethanolamine (DEA) buffer with an Auto Analyzer II apparatus was designed. The buffer used was 1.0 mol/1 DEA-HC1 buffer, pH 9.8 at 37 °C, containing 0.5 mmo1/1 of MgCl2 and 10 mmol/1 of substrate 4-nitro-phenyl-phosphate. The reaction time was about 3 min at 37 °C. The enzyme activity (U/1) was calculated by determining the amount of 4-nitrophenol formed in reaction. A sampling rate of 70 samples per hour can be used with good linearity up to 1000 U/1. The results obtained by the new continuousflow system were compared with those measured by the kinetic method according to the Scandinavian recommendation (10). A close correlation between the two methods was observed.