Toxicity of phospholipase C in rabbits

Ø. Hetland Research Institute for Internal Medicine, Rikshospitalet, Oslo, Norway; Department of Surgery, Institute for Clinical Medicine, University of Tromsø, Tromsø; Department of Biochemical Engineering, Rikshospitalet, Oslo, Norway

K. Warhuus Research Institute for Internal Medicine, Rikshospitalet, Oslo, Norway; Department of Surgery, Institute for Clinical Medicine, University of Tromsø, Tromsø; Department of Biochemical Engineering, Rikshospitalet, Oslo, Norway

K. E. Giercksky Research Institute for Internal Medicine, Rikshospitalet, Oslo, Norway; Department of Surgery, Institute for Clinical Medicine, University of Tromsø, Tromsø; Department of Biochemical Engineering, Rikshospitalet, Oslo, Norway

R. Aaslid Research Institute for Internal Medicine, Rikshospitalet, Oslo, Norway; Department of Surgery, Institute for Clinical Medicine, University of Tromsø, Tromsø; Department of Biochemical Engineering, Rikshospitalet, Oslo, Norway

H. Prydz Research Institute for Internal Medicine, Rikshospitalet, Oslo, Norway; Department of Surgery, Institute for Clinical Medicine, University of Tromsø, Tromsø; Department of Biochemical Engineering, Rikshospitalet, Oslo, Norway

Abstract

Tissue thromboplastin is the most potent physiological trigger of blood coagulation and is probably involved in the pathogenesis of several forms of intravascular coagulation. Phospholipase C from Bacillus cereus is an effective inhibitor of thromboplastin. As part of an investigation into the possible use of phospholipase C as a therapeutic or prophylactic agent in thrombosis and other forms of intravascular coagulation, we have previously studied its effect in rats. We now report on the toxicity in rabbits (along with some data on cats and monkeys). Estimated LD₅₀ for rabbits was about 0.45–0.65 mg/kg (as compared to 1.70 mg/kg for rats). No respiratory or circulatory changes were observed, but phospholipase C caused a significant increase of several plasma enzymes in rabbits.

Key-words:

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Toxicity of phospholipase C in rabbits

Information for

Open access

Opportunities

Help and information

Toxicity of phospholipase C in rabbits

Abstract

Reprints and Corporate Permissions

Academic Permissions

Related research

To cite this article:

Download citation

Information for

Open access

Opportunities

Help and information

Keep up to date

Your download is now in progress and you may close this window

Login or register to access this feature