Evaluation of microtitre plates for the routine assay of oestrogen and progesterone receptors in tissue extracts

Abstract

A time- and material-saving assay of oestrogen and progesterone receptors in tissue extracts using enzyme-linked immunosorbent assay quality microtitre plates and microtitre-compatible equipment is described. The results from simultaneous determinations in microtitre plates and in conventional tubes show the former to yield higher receptor values. Furthermore, the results of a large series of measurements in microtitre plates compare favourably with previously obtained results from assays in tubes.

Key Words:

• breast cancer
• steroid receptors