Regulation of Steroidogenesis by Cordyceps sinensis Mycelium Extracted Fractions with (hCG) Treatment in Mouse Leydig Cells

K. L. Wong Department of Anesthesia, Pain Management and Critical Care Medicine, China Medical University and Hospital, Taichung, Taiwan, ROC

E. Cheung So Department of Anesthesia, Chi-Mei Medical Center, Tainan, Taiwan, ROC; Institute of Basic Medical Science, National Cheng Kung University, Tainan, Taiwan, ROC

C. C. Chen Department of Anesthesia, Chi-Mei Medical Center, Tainan, Taiwan, ROC; Institute of Basic Medical Science, National Cheng Kung University, Tainan, Taiwan, ROC; Department of Cell Biology and Anatomy, National Cheng Kung University, Tainan, Taiwan, ROC

R. S. C. Wu Department of Anesthesia, Chi-Mei Medical Center, Tainan, Taiwan, ROC; Institute of Basic Medical Science, National Cheng Kung University, Tainan, Taiwan, ROC; Department of Cell Biology and Anatomy, National Cheng Kung University, Tainan, Taiwan, ROC

B.-M. Huang Department of Cell Biology and Anatomy, National Cheng Kung University, Tainan, Taiwan, ROCCorrespondence[email protected]

Abstract

The in vitro effect of extracted fractions of Cordyceps sinensis (CS) mycelium on hCG-treated testosterone production from purified normal mouse Leydig cells was examined. Different fractions extracted from CS (F1-water soluble polysaccharide, F2- water soluble protein and F3- poorly water soluble polysaccharide, and protein) were added to Leydig cells with hCG, and the production of testosterone was determined by radioimmunoassay (RIA). Testosterone productions stimulated by hCG in mouse Leydig cells were suppressed by F2 at 10 mg/ml and F3 at doses from 3 to 10 mg/ml, respectively. F2 and F3 at 10 mg/ml did inhibit dbcAMP-stimulated testosterone productions which indicated that F2 and F3 might affect steroidogenesis at the site after the formation of cyclic AMP. Finally, cycloheximide inhibited F2- and F3-treated mouse Leydig cell testosterone production.

KEYWORDS:

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Regulation of Steroidogenesis by Cordyceps sinensis Mycelium Extracted Fractions with (hCG) Treatment in Mouse Leydig Cells

Information for

Open access

Opportunities

Help and information

Regulation of Steroidogenesis by Cordyceps sinensis Mycelium Extracted Fractions with (hCG) Treatment in Mouse Leydig Cells

Abstract

Reprints and Corporate Permissions

Academic Permissions

Related research

To cite this article:

Download citation

Information for

Open access

Opportunities

Help and information

Keep up to date

Your download is now in progress and you may close this window

Login or register to access this feature