TARGETED EXPRESSION OF IP₃ SPONGE AND IP₃DSRNA IMPAIRES SUGAR TASTE SENSATION IN DROSOPHILA

Kazue Usui-Aoki Waseda University, School of Science and Engineering, Nishi-Tokyo, Japan; Current address: Chiba industry advancement center, JST-CREATE, Kisarazu, Japan

Ken Matsumoto Waseda University, School of Science and Engineering, Nishi-Tokyo, Japan

Masayuki Koganezawa Tohoku University, Graduate School of Life Sciences, Sendai, Japan

Sou Kohatsu Tohoku University, Graduate School of Information Sciences, Sendai, Japan

Kunio Isono Tohoku University, Graduate School of Information Sciences, Sendai, Japan

Hiroshi Matsubayashi Kyoto Institute of Technology, Drosophila Genetic Resource Center, Kyoto, Japan

Masa-Toshi Yamamoto Kyoto Institute of Technology, Drosophila Genetic Resource Center, Kyoto, Japan

Ryu Ueda National Institute of Genetics, Mishima, Japan

Kuniaki Takahashi National Institute of Genetics, Mishima, Japan

Kaoru Saigo The University of Tokyo School of Science, Tokyo, Japan

Katsuhiko Mikoshiba The Institute of Medical Science, The University of Tokyo, Tokyo, Japan

Daisuke Yamamoto Waseda University, School of Science and Engineering, Nishi-Tokyo, Japan; Tohoku University, Graduate School of Life Sciences, Sendai, JapanCorrespondence[email protected]

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Abstract

We evaluated the role of IP₃ in sugar taste reception in Drosophila melanogaster by inactivating the IP₃ signaling using genetic tools. We used the “IP₃ sponge,” composed of the modified ligand-binding domain from the mouse IP₃ receptor, which was designed to absorb IP₃ in competition with native IP₃ receptors. Another tool was a transgene that generates double-stranded RNA against IP₃ receptor mRNA. Both inhibitors diminished the sensitivity of flies to trehalose and sucrose, as estimated by behavioral assays and electrophysiological recordings from the sugar receptor cells. The result indicates that IP₃ signaling is indispensable for sugar reception in Drosophila.

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TARGETED EXPRESSION OF IP₃ SPONGE AND IP₃DSRNA IMPAIRES SUGAR TASTE SENSATION IN DROSOPHILA

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TARGETED EXPRESSION OF IP₃ SPONGE AND IP₃DSRNA IMPAIRES SUGAR TASTE SENSATION IN DROSOPHILA