185
Views
7
CrossRef citations to date
0
Altmetric
Original Article

Heat-induced changes in intracellular Na+, pH and bioenergetic status in superfused RIF-1 tumour cells determined by 23Na and 31P magnetic resonance spectroscopy

, , , &
Pages 141-158 | Received 21 Jul 2004, Accepted 11 Nov 2004, Published online: 09 Jul 2009
 

Abstract

The acute effects of hyperthermia on intracellular Na+ (Na), bioenergetic status and intracellular pH (pHi) were investigated in superfused Radiation Induced Fibrosarcoma–1 (RIF-1) tumour cells using shift-reagent-aided 23Na and 31P nuclear magnetic resonance (NMR) spectroscopy. Hyperthermia at 45°C for 30 min produced a 50% increase in Na, a 0.42 unit decrease in pHi and a 40–45% decrease in NTP/Pi. During post-hyperthermia superfusion at 37°C, pHi and NTP/Pi recovered to the baseline value, but Na initially decreased and then increased to the hyperthermic level 60 min after heating. Hyperthermia at 42°C caused only a 15–20% increase in Na. In the presence of 3 µM 5-(N-ethyl-N-isopropyl)amiloride (EIPA), an inhibitor of the Na+/H+ exchanger, the increase in Na during 45°C hyperthermia was attenuated, suggesting that the heat-induced increase in Na was mainly due to an increase in Na+/H+ anti-porter activity. EIPA did not prevent hyperthermia-induced acidification. This suggests that pHi is controlled by other ion exchange mechanisms in addition to the Na+/H+ exchanger. EIPA increased the thermo-sensitivity of the RIF-1 tumour cells only slightly as measured by cell viability and clonogenic assays. The hyperthermia-induced irreversible increase in Na suggests that changes in transmembrane ion gradients play an important role in cell damage induced by hyperthermia.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.