Glutathione and Lipid Peroxide Changes in Pseudoexfoliation Syndrome

Abstract

Purpose:To investigate the oxidative status of the aqueous humor of patients with pseudoexfoliation (PEX) syndrome. Methods: Aqueous humor samples obtained during cataract surgery of patients with PEX syndrome and normal age-matched control subjects were examined for changes in the levels of glutathione (GSH), glutathione disulfide (GSSG), and TBA reactive species (TBARS), products of lipid peroxidation. GSH, GSSG, and TBARS were determined by specific fluorescent assays. Results: Compared to normal controls, PEX syndrome aqueous humor samples showed a decrease of up to 28% of GSH concentration, and GSSG was increased up to 23%. The ratio of GSH/GSSG was 1.7-fold decreased in PEX syndrome samples. TBARS levels were increased by 100% in the PEX aqueous humor samples as compared to the controls. Conclusions: High levels of GSSG and TBARS indicate high oxidative stress, as well as the decrease in the ratio of GSH/GSSG. Our findings suggest a role for oxidation stress in the pathogenesis and the progression of PEX syndrome.

KEYWORDS:

• aqueous humor
• cataract
• glutathione
• lipid peroxidation
• pseudoexfoliation syndrome