Accumulation of FoxP3‐expressing CD4⁺CD25⁺ T cells with distinct chemokine receptors in synovial fluid of patients with active rheumatoid arthritis

Abstract

Objective: To explore the presence and characteristics of FoxP3‐expressing CD4⁺CD25⁺ regulatory T cells in synovial fluid (SF) of patients with active rheumatoid arthritis (RA).

Methods: The frequency and chemokine receptors expression profile of FoxP3‐expressing CD4⁺CD25⁺ regulatory T cells in SF and peripheral blood (PB) from RA patients and PB from healthy controls were investigated by flow cytometry using three‐ or four‐colour intracellular staining.

Results: The frequency of CD4⁺CD25⁺ FoxP3⁺ T cells was increased significantly in SF compared with paired PB from RA patients and PB from healthy controls (p<0.05). However, the frequency in PB from RA patients was significantly lower than in PB from healthy controls (p<0.05). Notably, CD4⁺CD25⁺FoxP3⁺ T cells in SF expressed increased levels of inflammation‐related trafficking chemokine receptors, such as CCR4, CCR5, and CXCR4.

Conclusion: There is an accumulation of FoxP3‐expressing regulatory T cells in RA SF, and such recruitment may be dependent on the distinct chemokine receptors expressed on regulatory T cells.