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Abstract
The distributions of the D1S80 alleles and genotypes in five endogamous populations (Lobanas, Jat Sikhs, Brahmins, Khatris and Scheduled castes) was determined by amplified fragment length polymorphism (AMP-FLP) technique. The distributions of the observed genotypes for the five populations conformed to Hardy-Weinberg expectations. Two alleles D1S80*18 and D1S80*24 were observed in all populations at frequencies similar to many Caucasian populations, but they showed significant inter-population variability within the region. Allele *24 varied from 33% (Scheduled caste) to 49% (Lobanas), while the allele *18 frequency was lowest in Lobanas (15%) and highest in Jat Sikhs (25%). There was significant overall heterogeneity among the populations studied for this locus. The heterozygosity, probability of exclusion, match probability and discrimination probability estimates demonstrate the usefulness of this locus for paternity and forensic purposes in Indian populations.