zHSF1 modulates zper2 expression in zebrafish embryos

ABSTRACT

HSF1 is a transcription factor that plays a key role in circadian resetting by temperature. We have used zebrafish embryos to decipher the roles of zHsf1, heat and light on zper2 transcription in vivo. Our results show that heat shock (HS) stimulated zper2 expression in the dark but has no cumulative effect combined with light. After light exposition, zper2 expression was 2.7 fold increased threefold in the hsf1-morphants in comparison to control embryos. Our results show that zHsf1 plays a positive role in HS-driven expression of zper2 in the dark but seems to act as an attenuator in the presence light.

KEYWORDS:

• crispants
• zper2
• zHSF1
• zebrafish
• morpholino knockdown

Acknowledgements

This work was supported by the INSERM, the Ligue Contre le Cancer du Grand Est (CCIR-GE), the Conseil Régional de Bourgogne and the Université de Bourgogne Franche-Comté. and the Université de Bourgogne Franche-Comté.

Declaration of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this article.