The anti-inflammatory effects of curcumin on renal ischemia-reperfusion injury in rats

Abstract

The present study aimed to explore the protective mechanism of curcumin-precondition in renal ischemia-reperfusion (I/R) injury. Thirty male SD rats were randomly equally divided into Sham, IRI, and Curcumin groups (n = 10). The model of IRI was induced by clamping the left renal artery for 45 min followed by 24 h reperfusion with the contralateral nephrectomy. ELISA was used to examine the expression of Cr, BUN, IL-8, TNF-α, and IL-6 in the serum; RT-PCR was used to detect the mRNA level of IL-8,TNF-α, and IL-6 in the kidney; The morphology of kidney was examined by Periodic Acid-Schiff stain (PAS). The expression of JAK2, p-JAK2, STAT3, p-STAT3, p65, and p-p65 in kidney were detected by Western blotting. The result displayed that Curcumin pretreatment can significantly increase the expression of p-JAK2 and p-STAT3 and reduce the expression of Cr, BUN, IL-8, TNF-α, IL-6, and p-p65. In addition, Curcumin can attenuate the kidney pathological injury and have no effect on the expression of JAK2, STAT3, and p65. Our findings showed the protective effect of Curcumin in I/R injury is associated with suppressing NF-κB mediating inflammation by activating JAK2/STAT3 signal pathway.

Keywords:

• Curcumin
• renal ischemia reperfusion injury
• inflammation

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

The project received financial support from National Natural Science Foundation of China (No. 81500575, 81401362); Scientific Research Foundation of Sichuan Provincial Health and Family Planning Commission (No. 18PJ365, 18PJ390); Foundation of Sichuan Provincial People's Hospital (No. 2017LY11).