Effect of Perfusion and Preservation on IL1-β, IL-6, and IL-10 Production in Murine Lung

Abstract

We evaluated the production of the interleukins (ILs) IL1- &#103 , IL-6, and IL-10 in both the vasculature and pulmonary tissue before and after 24 h of lung preservation. The cardiopulmonary blocs of 21 Balb-c mice were divided into three study groups (7 mice/group) and were flushed through the pulmonary artery with Krebs-Henseleit buffer (K-Hb) at 4°C at a rate of 0.2 mL/min as follows: Group 1, lung washout: lungs were flushed until pulmonary effluent was clear. Group 2, perfusion: After lungs were flushed until pulmonary effluent was clear, lungs were perfused during 30 min. Group 3, preservation: Lungs were flushed until pulmonary effluent was clear, and the cardiopulmonary bloc was preserved immersed into (K-Hb) at 4°C. After 24 h of preservation, lungs were reperfused during 30 min. In all study groups the caudal lobe from the left lung was taken for microscopical study; all other lobes were homogenized with (K-Hb) and the supernatant was obtained. IL-1 &#103 , IL-6, and IL-10 production in lung effluents (washout, perfusion, and reperfusion) and in lung tissue were measured by enzyme-linked immunosorbent assay (ELISA). In the lung effluent, there was no statistical difference between IL-1 &#103 and IL-6 concentrations. In all study groups, IL-10 production was significantly higher than IL-1 &#103 and IL-6 levels. IL-10 level was lowest in the 24-h preservation group when it was compared to the other groups. In group 1, there was a negative correlation ( r =-.599, p < .05) between IL-1 &#103 and IL-10. In pulmonary tissue, IL-1 &#103 was higher in group 2 when compared to groups 1 ( p = .001) and 3 ( p = .002), and it was significantly lower in group 3. IL-10 was lower in group 1 when compared to groups 2 ( p = .001) and 3 ( p = .004). In groups 1 and 2, IL-1 &#103 was significantly higher than IL-6 and IL-10. In group 3, IL-10 was higher than IL-1 &#103 ( p = .0001) and IL-6 ( p = .0001). Correlation of effluent/tissue index with histological findings showed a negative correlation between IL-10 effluent/tissue relation and inflammation ( r =-.68, p < .01). In conclusion, the main cytokine found in lung effluents was IL-10, followed by IL-6 and IL-1 &#103 . On the other hand, cytokine concentration in lung tissue homogenates was mainly due to the presence of IL-1 &#103 . However, this cytokine shows a significant reduction in lung tissue after prolonged preservation.

• Historical Changes
• Il-1BETA
• Il-6
• Il-10
• Lung Perfusate
• Mice
• Perfusion-ISCHEMIA-REPERFUSION
• Pulmonary Artery Flush
• Pulmonary Tissue