Effects of propofol on IGF-1 activity and cell behaviour in the GES 1 mucosal cell model

Abstract

Propofol is an important and widely used anaesthetic drug in the clinic. Many works have shown that propofol has important biological functions except as an anaesthetic. In the current study, we mainly explored the effect of propofol on the biological activity of IGF-1, which is an important growth factor involved in regulating the growth and development of the stomach. Here, we explored the effect of propofol on the biological activity of IGF-1 in a GES-1-cell model. We found that propofol affected the biological activity of IGF-1. It not only reduces IGF-1/IGF-1R signalling but also changes IGF-1R cell characteristics. We further explored the mechanism by which propofol affected IGF-1 activity. Through a series of experiments, we found that propofol affected the stability of membrane-localised IGF-1R. It also affects the recycling of the IGF-1R receptor Propofol can affect the degradation of IGF-1R by changing the endocytosis of IGF-1R. In short, the current study found that propofol affected the biological activity of IGF-1, which laid the foundation for related research.

Keywords:

• Propofol
• signalling pathway
• IGF-1R
• IGF
• intestinal cells

Acknowledgments

The authors would like to thank Dr. Zhang for CLSM.

Author contributions

Chai Hua and Jin Xiayun designed the experiments; Peng Wenyong, Zhu Zhongquan and Xiong Chang performed the laboratory work; and Chai Hua wrote the manuscript. All authors were involved in analysing the data and/or providing editorial comments/corrections to the final article.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This work is financially supported by Jinhua Science and Technology Plan Project Social Development Key Project [2019-3-013].