Effects of three pro-nuclei (3PN) proportion incidence on clinical outcomes of patients with lower retrieved oocytes in the fresh cleavage-stage embryo transfer (ET) cycles

Abstract

Purpose: To analyze the three pro-nuclei (3PN) incidence on clinical outcomes of patients with lower retrieved oocytes in the fresh cleavage-stage embryo transfer (ET) cycles.

Methods: This study included 1200 fresh cleavage-stage ET cycles from January 2013 to June 2015. The patients were divided into 3PN = 0% (773 cycles) and 3PN > 0% (427 cycles) group. Main outcomes compared were fertilization, cleavage, normal fertilization, good quality embryo, implantation, clinical pregnancy, and early abortion rate.

Results: We observed that there was no significant difference in female's age, the number of retrieved oocytes, the number of transferred embryos, the number of good quality embryos, endometrial thickness, infertile time, basal serum follicle-stimulating hormone, and E2 value between two groups (p > 0.05). The fertilization (89.43 versus 83.90%, p < 0.001) and cleavage (98.34 versus 97.19%, p = 0.048) rates were significantly higher in 3PN > 0% than 3PN = 0% group. However, the normal fertilization (70.05 versus 50.67%, p < 0.001), good quality embryos (37.11 versus 26.47%, p < 0.001), and clinical pregnancy (49.81 versus 43.79%, p = 0.046) rates were significantly higher in 3PN = 0% than 3PN > 0% group. The implantation (35.88 versus 33.78%, p = 0.333) and early abortion (8.83 versus 10.70%, p = 0.474) rates were not significantly different between two groups.

Conclusion: 3PN incidence might make a negative effect on clinical outcomes for patients with lower retrieved oocytes in the fresh cleavage-stage ET cycles.

Chinese abstract

目的: 为分析在新鲜的卵裂期胚胎移植周期中三原核 (3PN) 发病率对低取卵患者临床结果的影响。

方法: 本研究包括从2013年1月到2015年6月共1200例 。将患者分为 3PN =0% (773个周期) 3PN> 0% (427个周期) 组。主要比较两组的受精、分裂、正常受精、优质胚胎、种植、临床妊娠和早期流产率。

结果: 我们观察到, 两组之间在女性年龄、获卵数、移植胚胎数、优质胚胎数、子宫内膜厚度, 不孕的时间、卵泡刺激素和E2值均无显著性差异 (P > 0.05) 。受精率 (89.43比83.90%, P＜0.001) 和分裂率 (98.34比97.19%, P = 0.048) 是3PN >0%组显著高于3PN =0%组。然而, 正常受精率 (70.05%比50.67%, P＜0.001) , 优质胚胎率 (37.11% 比26.47%, P＜0.001) , 与临床妊娠 (49.81% vs 43.79%, P = 0.046) 率均是3PN =0%显著高于3PN >0%组。植入 (35.88%比33.78%, P = 0.333) 和早期流产 (8.83%与10.70%, P = 0.474) 两组间无显著性差异。

结论: 在新鲜的卵裂期胚胎移植周期中3PN发病可能对低取卵患者的临床结果产生负面影响。

Keywords:

• Cleavage-stage embryo
• clinical outcomes
• 3PN

Declaration of interest

The authors report no conflicts of interest.