Polymorphism of the THOC5 of the transcription/export multiprotein complex and its correlation with the lipid and metabolic profile in middle-aged women

Abstract

The transcription/export complex (TREX) is formed by a core called THO. These are necessary for the transcription and packaging of messenger RNA and its subsequent nuclear exportation. Studies have correlated THO-specific polymorphisms with abnormalities of HDL-C metabolism. To correlate lipid and metabolic parameters with the genetic variants of the rs8135828 polymorphism of the THOC5 gene in middle-aged women. DNA was extracted from the whole blood of 183 women aged 40–65 and tested for the rs8135828 polymorphism of the THOC5 gene using real-time PCR. HDL-C, LDL-C, triglyceride, and total cholesterol levels, as well as other metabolic parameters, were correlated with the polymorphism genotypes: GG, AG, and AA. Mean age of women was 50.6 ± 6.3 years, 54.6% were postmenopausal and 16.4% had the metabolic syndrome. GG was the most frequently determined genotype (62.3%). There were no differences in lipid levels according to genotypes; although there was a trend for lower HDL-C levels for the AA and AG + AA genotypes. Those with the AG and AG + AA genotypes displayed significantly higher glucose levels (p = .02 and p = .002, respectively); with a trend toward a higher metabolic syndrome prevalence and increased abdominal perimeters in both variants (AG and AG + AA). The AG genotype was related to higher glucose levels but not with abnormal lipid parameters. There is a need for more research in this regard.

摘要

转录/导出复合体（TREX）由称为THO的核心形成, 这些对于信使RNA的转录和包装以及随后的核输出都是必需的, 研究已证实THO特异性多态性与HDL-C代谢异常相关。 为了研究脂质和代谢参数与中年妇女THOC5基因的rs8135828多态性的遗传变异的相关性, 从183位40-65岁妇女的全血中提取DNA, 并使用实时PCR检测THOC5基因的rs8135828多态性。HDL-C、LDL-C、甘油三酸酯和总胆固醇水平以及其他代谢参数与多态性基因型相关：GG, AG和AA型。妇女的平均年龄为50.6±6.3岁, 绝经后为54.6％, 代谢综合征为16.4％。GG是最常见的基因型（62.3％）, 根据基因型, 血脂水平没有差异, 尽管AA和AG + AA基因型的HDL-C水平有降低的趋势。那些具有AG和AG + AA基因型的人显示出明显更高的葡萄糖水平（分别为p = 0.02和p =0.002）；两种变型（AG和AG + AA）的代谢综合征患病率有上升趋势, 腹围也相应增加。AG基因型与较高的葡萄糖水平有关, 但与异常的脂质参数无关, 在这方面还需要更多的研究。

The Chinese abstracts are translated by Prof. Dr. Xiangyan Ruan and her team: Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China.

Keywords:

• Lipids
• metabolic
• middle age
• women
• transcription/export multiprotein complex
• THO complex
• polymorphisms

Acknowledgments

The authors would like to thank women who participated in this initiative.

Disclosure statement

The authors report no potential conflicts of interest.

Additional information

Funding

This research was partially supported by the Sistema de Investigación y Desarrollo and the Vice-Rectorado de Investigación & Postgrado of the Universidad Católica de Santiago de Guayaquil, Guayaquil, Ecuador, through grant No. SIU-318–853-2014 (The Omega II, Women’s Health Project 2014).