Abstract
The aim of this study was to evaluate the effect of virus infection on estradiol (E2) production in human ovarian granulosa cells. Polyriboinosinic polyribocytidylic acid [Poly (I: C)], a synthetic analog of viral double stranded RNA that can be recognized by Toll like receptor 3 (TLR3), was used to imitate virus infection. Granulosa cells (GCs) obtained from patients undergoing in vitro fertilization and embryo transfer (IVF-ET) were cultured in vitro and treated with Poly (I: C), FSH, or both. Concentration of E2 was assayed by electrochemiluminescence. The mRNA and protein expression of TLR3 and aromatase were determined by real-time quantitative PCR (qPCR) and Western blot, respectively. The results showed that expression of TLR3 mRNA was significantly increased after Poly (I: C) stimulation. Poly (I: C) decreased E2 synthesis in FSH-treated GCs. Poly (I: C) inhibited the expression of aromatase in FSH-treated GCs. This study demonstrated that Poly (I: C) inhibits the synthesis of estradiol by granulosa cells under the stimulation of FSH, which might contribute to disturbance of follicular development and ovulation.
摘要
本研究旨在评估病毒感染对人卵巢颗粒细胞分泌雌二醇(E2)的影响。多聚次黄嘌呤胞嘧啶核苷酸[Poly(I∶C)]是一种人工合成的可被Toll样受体3 (TLR3)识别的病毒双链RNA模拟物, 用其模拟病毒感染。对来自体外受精和胚胎移植(IVF-ET)患者的颗粒细胞(GCs)进行体外培养, 并用Poly (I∶C)、FSH或两者共同对其进行处理。用电化学发光法测定E2浓度。分别用实时定量PCR(qPCR)和Western blot测定TLR3和芳香化酶的mRNA和蛋白表达。结果显示:Poly(I∶C)刺激后TLR3 mRNA的表达显著增加。Poly(I∶C)使FSH处理的GCs其E2合成减少。Poly(I∶C)抑制FSH处理的GCs内芳香化酶的表达。研究显示Poly(I∶C)抑制经FSH刺激的颗粒细胞的雌二醇合成, 这或许能破坏卵泡的发育及排卵。
The Chinese abstracts are translated by Prof. Dr. Xiangyan Ruan and her team: Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China.
Disclosure statement
All authors declare that they have no competing interests.