Effect of methotrexate on embryonal implantation: an experimental rat model

Abstract

The aim of this study is to investigate the methotrexate (MTX) in rat embryonal implantation and its association with Glycodelin A (GdA) and Mucin-1 (MUC-1) expression. For this purpose, 32 pregnant rats were divided into four equal groups: non-pregnant rats in group I (n = 8, control) and pregnant rats in group III (n = 8) were injected intraperitoneal with single dose of normal saline, non-pregnant rats in group II (n = 8) and pregnant rats in group IV (n = 8) were given 0.2 mg i.m. injection of MTX before three months of pregnancy. The dams were killed on 5th day of gestation and uterine horn samples were removed. Following dissection and routine histological preparation, immunohistochemical analysis was carried out. During immunohistochemical examination of the tissue samples prepared from the control and experimental groups, a statistically significant difference was observed between the groups in the luminal–glandular–decidualized epithelium of the uterus with GdA and MUC-1. Finally, in light of our findings, MTX adversely affected the expression of two molecules in Wistar Albino rats embryonal implantation model.

摘要

本研究的目的是研究大鼠胚胎植入中甲氨蝶呤（MTX）及其与胎盘蛋白A （GdA）和粘蛋白1 （MUC1）表达的关系。为此, 将32只怀孕大鼠分成四组：未怀孕的老鼠在I组（n=8、对照组）和怀孕的大鼠在第III组（n=8）腹腔注射单剂量的生理盐水；未怀孕大鼠在第II组（n=8）和怀孕大鼠在第IV组（n=8）于怀孕前三个月肌注0.2mg MTX。在妊娠第5天杀死幼崽, 取出子宫角标本。在解剖和常规组织学准备后, 进行免疫组织化学分析。对对照组和实验组的组织标本进行免疫组化检查, GdA和MUC1组间子宫管腔腺蜕膜上皮组织差异有统计学意义。最后, 根据我们的发现, MTX对Wistar白化大鼠胚胎植入模型中两个分子的表达产生了不利影响。

Keywords:

• Embryonal implantation
• methotrexate
• Mucin-1
• Glycodelin A

Acknowledgements

All applicable institutional guidelines for the care and use of animals were followed.

Disclosure statement

No potential conflict of interest was reported by the author(s).