Abstract
Investigation of the bone marrow as the main compartment of hematopoiesis is critical in many research fields. Here, we adapted a centrifugation-based method for the isolation of murine bone marrow and compared it to the traditional flushing method. Analysis of primary hematopoietic stem cells, immune cells, and megakaryocytes revealed a comparable distribution of cellular (sub)populations. Furthermore, in vitro differentiated megakaryocytes displayed unaltered proplatelet formation. Strikingly, bone marrow isolation by centrifugation was considerably faster than the flushing method and significantly increased the cell yield. Thus, the centrifugation-based isolation method is highly suitable for the study of murine bone marrow cells.
Acknowledgements
We thank Lou M. Wackerbarth for help with megakaryocyte cultivation experiments.
Authorship Contribution
TH and CG performed experiments, analyzed data, and wrote the manuscript; MLM established the immune cell flow cytometry in our laboratories; IP and DS designed and supervised research, and wrote the manuscript.
Declaration Of Interest
DS is on the editorial board of Platelets.