Cellular kinetics of hematopoietic cells with Sfpi1 deletion are present at different frequencies in bone-marrow and spleen in X-irradiated mice

ABSTRACT

Purpose

Several past studies using a mouse model of radiation-induced AML (rAML) have shown that hemizygous deletion of the Sfpi1 gene (HDSG) is an initiating event for the development of rAML. In this study, we examined the difference in frequency of HDSG in hematopoietic stem cells (HSCs) Rich hematopoietic Cell population (HRCs) from bone marrow (BM) and spleen of C3H mice irradiated with 3 Gy X-rays.

Materials and methods

8-weeks old male C3H mice were irradiated 3Gy of whole body X-ray (1 Gy/min) and mice were sacrificed at 1, 4, 8, and 26 weeks. Then, HSPCs were isolated from BM of femur and spleen, the frequency of HRCs with Sfpi1 gene deletion was analyzed by fluorescence in situ hybridization (FISH).

Results and conclusions

The frequency of HRCs with HDSG in both BM and spleen was increased 1 week after X-irradiation. Then, the frequency of HRCs with HDSG in BM showed a gradual decrease from 4 to 26 weeks, whereas HRCs with HDSG in spleen remained high, even at 26 weeks after X-irradiation. HDSG is less likely to be eliminated, particularly in the spleen, after X-irradiation. The spleen as well as BM of the femur may be major sites of rAML development.

KEYWORDS:

• Word
• radiation-induced acute myeloid leukemia (rAML)
• Sfpi1 gene
• hematopoietic cells

Acknowledgments

This work was supported by the Interdisciplinary Project on Environmental Transfer of Radionuclides (No. HY-18-5).

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Notes on contributors

Reo Etani

Reo Etani, PhD, is an Assistant Professor at Laboratory of Environmental Health Science, Oita University of Nursing and Health Sciences.

Mitsuaki Ojima

Mitsuaki Ojima, PhD, is an Associate Professor at Laboratory of Environmental Health Science, Oita University of Nursing and Health Sciences.

Kentaro Ariyoshi

Kentaro Ariyoshi, PhD, is an Associate Professor at the Integrated Center for Science and Humanities, Fukushima Medical University.

Yohei Fujishima

Yohei Fujishima, PhD, is an Assistant Professor at Department of Radiation Biology, Tohoku University Grauduate School of Medicine.

Michiaki Kai

Michiaki Kai, PhD, is a Professor at Laboratory of Environmental Health Science, Oita University of Nursing and Health Sciences.