An experimental approach to comprehend the influence of platelet rich growth factors on spermatogenesis

Abstract

Leukocyte-poor platelet-rich plasma (LP-PRP) has a potential influence on tissue regeneration by stimulating the proliferation and differentiation of progenitor cells. The aim of this work is the morphological assessment of spermatogenesis under the influence of growth factors after electron irradiation.

Materials and methods

Experimental animals (n = 135) were divided into 5 groups: I – control (n = 10); II – 2IR (n = 35; 2 Gy); III – 2IR + LP-PRP + IGF-1 (n = 30); IV – 2IR + LP-PRP (n = 30); V – LP-PRP (n = 30).

Results

Electron irradiation reduces the number of germ cells in comparison with the control group. After injection of LP-PRP + rhIGF-1 significantly increased the number of germ cells, Sertoli and Leydig cells, the height of germinal epithelium, area and diameter of seminiferous tubules.

Conclusion

LP-PRP + rhIGF-1 has a normalizing effect on structural and functional disorders of the testis caused by electron irradiation.

Keywords:

• Infertility
• irradiation
• seminiferous tubules
• spermatogenesis
• apoptosis

Declaration of interest statement

The authors have no conflict of interests to declare.

Additional information

Notes on contributors

Grigory A. Demyashkin

Grigory A. Demyashkin, PhD, MD, Head of the Department of Radiological pathomorphology of “NMRC of Radiation”, Leading researcher Institute of Translation medicine of Sechenov University; member of the European Association of Urology, section of Andrological Urology (ESAU).

Tatyana G. Borovaya

Tatyana G. Borovaya, PhD, MD, Leading researcher Laboratory of anatomy of microorganisms of N.F. Gamaleya Federal Research Center for Epidemiology & Microbiology.

Yulia Yu. Andreeva

Yulia Yu. Andreeva, PhD, MD, Professor of the Department of Pathological Anatomy of Russian Medical Academy of Continuous Professional Education.

Andrey A. Nedorubov

Andrey A. Nedorubov, Head of Institute of translation medicine of Sechenov University.

Yulia Yu. Stepanova

Yulia Yu. Stepanova, member of the Institute of clinical medicine named after N.V. Sklifosovsky of Sechenov University.

Matvey A. Vadyukhin

Matvey A. Vadyukhin, member of the Institute of clinical medicine named after N.V. Sklifosovsky of Sechenov University.

Vladimir I. Shchekin

Vladimir I. Shchekin, member of the Institute of clinical medicine named after N.V. Sklifosovsky of Sechenov University.

Sergey N. Koryakin

Sergey N. Koryakin, PhD, Head of Department of Radiation Biophysics of ²A.F. Tsyba Medical Radiological Research Center – a branch of the Federal State Budgetary Institution "National Medical Research Center of Radiology".

Peter V. Shegay

Peter V. Shegay, PhD, oncologist; Head of the Center of Innovational Radiological and Regenerative Technologies of “NMC of Radiation”.

Andrey D. Kaprin

Andrey D. Kaprin, PhD, MD, oncologist; General Director of “NMC of Radiation”; Head of the Department of Oncology; Аcademician of the Russian Academy of Sciences.