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Summary
The effect of x-radiation on the graft-versus-host reaction produced by injecting spleen cells of the parental strain into the F1 hybrid cross between two inbred strains of rat has been studied. Two separate measurements of the intensity of this reaction have been used: the enlargement of the spleen, and the degree of dilation of the peripheral blood vessels.
This reaction is increased by irradiating the hybrid recipients prior to injecting parental-strain spleen cells from normal animals. The survival has been determined of irradiated parental-strain spleen cells obtained from rats that were alive at the time of irradiation and also of cells obtained from rats killed a few minutes before irradiation.
The results suggest that spleen cells which take part in the graft-versus-host reaction are well oxygenated in the living rat and have approximately the same sensitivity as mouse lymphoid cells which take part in similar homograft reactions. There appears to be no difference in the sensitivity of cells irradiated in vivo and in vitro under the same conditions of oxygenation. However, spleen cells taking part in the homograft reaction are less sensitive than spleen cells that produce antibody.