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Summary
The production of aerobic lactate by suspensions of rat thymocytes incubated at 37° was considerably increased after x-irradiation. Intracellular concentrations of intermediates and cofactors of glycolysis were measured at different incubation times. Fructose-1,6-diphosphate showed a remarkable increase during incubation. Crossover plot of changes in the levels of intermediates and changes in mass-action ratios for glycolytic reactions have clearly demonstrated the enhanced activity of phosphofructokinase after irradiation. The possible correlation between the activated phosphofructokinase and lactate production was discussed.