Gamma-irradiated Chymotrypsin-like Proteins

Summary

The radiation yields of unfolding (G_conf) determined by the method of tryptophan fluorescence coincide with the radiation yields of proteolytic inactivation (G_in) for chymotrypsin-like (CT-like) enzymes on irradiation in air, both in solution and in the dry state with further dissolution at pH 7. It can be supposed that the unfolding is the main process determining the proteolytic γ-inactivation of CT-like enzymes. It was also shown that the transition of chymotrypsin and trypsin γ-irradiated at acid pH to neutral pH is an additional action, leading to unfolding of part of the molecules.