Comparison of Three Mammalian Cell-lines with Respect to Their Sensitivities to Hyperthermia, γ-rays and U.V.-radiation

Summary

Three types of cultured mammalian cells, from a rat ureter carcinoma (RUC-2), from a mouse mammary carcinoma (M8013), and from a Chinese hamster lung cell-line (CH-V79), have been compared with respect to various treatments. Using cloning assay, the sensitivity of these cell-lines was measured for ionizing radiation, U.V.-light and for temperatures of 41°, 42°, 43°, 44° and 45°C. RUC-2 cells are the least sensitive to these treatments, except for 45°C. M8013 is sensitive to γ-rays and U.V.-irradiation. CH-V79 is sensitive to γ-radiation and hyperthermia. Since sensitivity to one type of treatment does not correlate with similar susceptibility to the others, it is concluded that different mechanisms may be involved in inactivation by these treatments.