Radical Formation in Single Crystals of Hypoxanthine·HCl·H₂O, Inosine, and the Disodium Salt of 5′-inosine-monophosphate

Summary

Radical formation in single crystals of hypoxanthine · HCl · H₂O, inosine and Na₂-5′-IMP · (7·5 H₂O) by X-irradiation has been studied using electron-spin-resonance spectroscopy at 9·5 and 35 GHz. In all crystals both H-addition radicals at position C2 and C8 of the purine ring are found. The coupling constants of these two radicals are different and depend strongly on the protonation state of the base. INDO-calculations indicate that the C8-radical is protonated at O6. In Na₂-5′-IMP OH-addition radicals at position C2 of the purine ring are formed. Electron adduct radicals are found in the neutral and the N7-protonated base after X-irradiation at 77 K. In Na₂-5′-IMP no electron adduct is formed but a radical which probably is the cation. In hypoxanthine · HCl · H₂O a radical could be observed after X-irradiation at 77 K, which results from addition of a Cl⁻ to the nitrogen N1.