Effect of Oxygen and Misonidazole on Radiation Damage in Biologically Active DNA Dissolved in a Bacterial Extract. Influence of Cytochrome C

M.V.M. Lafleur Physics Laboratory of the Vrije Universiteit, Department of Biophysics, De Boelelaan 1081, 1081, HV Amsterdam, The Netherlands

E.J. Pluijmackers-Westmijze Physics Laboratory of the Vrije Universiteit, Department of Biophysics, De Boelelaan 1081, 1081, HV Amsterdam, The Netherlands

H. Loman Physics Laboratory of the Vrije Universiteit, Department of Biophysics, De Boelelaan 1081, 1081, HV Amsterdam, The Netherlands

Summary

The effect of radiation on biologically active DNA, dissolved in a bacterial extract was studied. The results show that oxygen affects DNA inactivation for both double-stranded (RF) and single-stranded ϕX174 DNA. Misonidazole induces an increased radiosensitivity in this system as found for single-stranded DNA. These effects disappear with ageing of the extract, but can be recovered by the addition of 10⁻⁶ M cytochrome c.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Effect of Oxygen and Misonidazole on Radiation Damage in Biologically Active DNA Dissolved in a Bacterial Extract. Influence of Cytochrome C

Information for

Open access

Opportunities

Help and information

Effect of Oxygen and Misonidazole on Radiation Damage in Biologically Active DNA Dissolved in a Bacterial Extract. Influence of Cytochrome C

Summary

Reprints and Corporate Permissions

Academic Permissions

Related research

To cite this article:

Download citation

Information for

Open access

Opportunities

Help and information

Keep up to date

Your download is now in progress and you may close this window

Login or register to access this feature