Effects of Gamma-irradiation on the Erythrocyte Membrane: ESR, NMR and Biochemical Studies

A. Cantafora Laboratorio di Metabolismo e Biochimica Patologica, Sezione Sanità, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161, Roma, Italy

M. Ceccarini Laboratorio di Biologia Cellulare, Sezione Sanità, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161, Roma, Italy

L. Guidoni Laboratorio di Fisica, and Istituto Nazionale di Fisica Nucleare, Sezione Sanità, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161, Roma, Italy

F. Ianzini Laboratorio di Metabolismo e Biochimica Patologica, Sezione Sanità, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161, Roma, Italy

M. Minetti Laboratorio di Biologia Cellulare, Sezione Sanità, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161, Roma, Italy

V. Viti Laboratorio di Fisica, and Istituto Nazionale di Fisica Nucleare, Sezione Sanità, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161, Roma, Italy

Summary

The effects of gamma-irradiation on resealed erythrocyte ghosts have been examined with different techniques. Phospholipid analysis reveals peroxidative damage on the polyunsaturated chains of phosphatidylethanolamine. Gel electrophoresis and ESR measurements indicate modifications of the cytoskeletal proteins. ³¹P Nuclear magnetic resonance data show bilayer modifications that can be interpreted as changes in lipid—protein interactions. The overall picture from the present results favours interaction between lipids and proteins in the inner monolayer of the membrane.

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Effects of Gamma-irradiation on the Erythrocyte Membrane: ESR, NMR and Biochemical Studies

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Effects of Gamma-irradiation on the Erythrocyte Membrane: ESR, NMR and Biochemical Studies

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