The Induction of Micronuclei in Human Lymphocytes By Low Doses of Radiation

Summary

The appearance of micronuclei (MN) is delayed with respect to cell division in populations of irradiated human lymphocytes, so that the length of time in culture, as well as the number of divisions, is a factor in MN assays. Using two assays that control for cell kinetics, we measured the yield of cells with MN exposed to graded doses of ⁶⁰Co gamma rays and 90 KVP X-rays. The yields showed a non-linear increase with dose. They can be represented by two straight lines: the one in the range below 0·15 Gy has a slight slope, the other in the range above 0·15 Gy has a significantly greater slope. The radical scavengers cysteamine and glycerol, which reduced the MN yields sharply at 3 Gy, were less effective at 0·3 Gy, indicating that terminal deletions arising from the direct ionization of DNA are a major source of the MN induced by low radiation doses. It is likely that the non-linear dose response is due to the saturation of a DNA repair process.