Studies of Ionizing Radiation as a Promoter of Neoplastic Transformation in Vitro

Summary

The induction of malignant transformation was examined in a standard promotion protocol in which BALB/3T3 cells were incubated continuously with tritiated water (³HOH) following acute treatment with various doses of either X-rays of benzo(a)pyrene (BP). In no case was there any evidence that protracted exposure to ionizing radiation from ³HOH enhanced the yield of transformants induced by the primary carcinogen over that predicted if the effects of the two agents were additive.