Induction of Chromosome Aberrations in Human Lymphocytes by Monochromatic X-rays of Quantum Energy between 4·8 and 14·6 keV

Summary

The induction of chromosome aberrations was studied in human peripheral blood lymphocytes irradiated in vitro with synchrotron-produced monochromatic soft X-rays of quantum energy in a range between 4·8 and 14·6 keV. These X-rays were more effective in producing chromosome aberrations (dicentrics and rings) than ⁶⁰Co γ-rays. The efficiency increased with increasing LET of the photoelectrons and their associated Auger electrons, reaching a maximum at a track average LET of around 4 keV/µm, and tended to decrease or become rather refractory with further increase of LET. This unique LET dependency was consistent with the dual nature of chromosome aberration formation, and interpreted as a reflection of a limited range of photoelectrons as compared with the size and intranuclear geometry of the elemental chromatin fibres as vehicles of damage interaction.