Bone Marrow from Balb/c Mice Radiocontaminated with ²⁴¹Am in Utero Shows a Deficient in Vitro Haemopoiesis

Summary

Radiation damage from ²⁴¹Am to bone marrow cells was manifest in long-term bone marrow cultures (LTC) from offspring of mice radiocontaminated at the 14th day of gestation (119, 479, 803, 1754 kBq ²⁴¹Am/kg). Offspring were reared by their own contaminated mother for 3 weeks postnatal. LTC from these offspring were less able to support in vitro CFC proliferation than control LTC from non-contaminated offspring. This radiation damage persisted 71 weeks after radiocontamination in utero. Using this in vitro culture system, damage was observed at lower doses if ²⁴¹Am contamination occurred at foetal than at adult ages. Radiation damage was observed only using LTC, while the haemopoietic stem cell concentration (CFU-S, in vitro CFC) and the stromal stem cell concentration (CFU-F) from marrow in situ were not impaired after ²⁴¹Am radiocontamination in utero. After culturing LTC in 25 per cent FCS and recharging the stromal adherent layer with bone marrow cell suspensions originating either from control offspring or from offspring contaminated with ²⁴¹Am in utero, some evidence was found that the proliferation capacity of the haemopoietic cells was diminished. However, the nature of effects on the stromal elements is currently somewhat equivocal. Following in utero contamination the stromal adherent cells appeared to support better the production of in vitro CFC.