Characterization of Radiation-induced Damage in d(TpApCpG)

C.R. Paul Department of Biophysics, Roswell Park Memorial Institute, 666 Elm Street, Buffalo, New York, 14263, USA

E.E. Budzinski Department of Experimental Therapeutics, Roswell Park Memorial Institute, 666 Elm Street, Buffalo, New York, 14263, USA

A. Maccubbin Department of Biophysics, Roswell Park Memorial Institute, 666 Elm Street, Buffalo, New York, 14263, USA

J.C. Wallace Department of Experimental Therapeutics, Roswell Park Memorial Institute, 666 Elm Street, Buffalo, New York, 14263, USA

H.C. Box Department of Biophysics, Roswell Park Memorial Institute, 666 Elm Street, Buffalo, New York, 14263, USA

Summary

The radiation chemistry of the oligomer d(TpApCpG) X-irradiated in aqueous solution containing glutathione was studied. Four products were isolated by HPLC and characterized by NMR spectroscopy. Two of the major products are isomers of a 5-hydroxy-5,6-dihydrothymine modification of d(TpApCpG). A dihydrothymine modification is also formed. The other major product is a result of strand scission. These products are different from the major products identified previously in a study of d(TpApCpG) X-irradiated in oxygenated solution. The effect of specific radiation-induced lesions on the behaviour of d(TpApCpG) as substrate to a spleen phosphodiesterase-micrococcal nuclease combination of enzymes and to nuclease P1 was studied. These enzymes are of interest because they are used in postlabelling assays of DNA damage.

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Characterization of Radiation-induced Damage in d(TpApCpG)

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Characterization of Radiation-induced Damage in d(TpApCpG)

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