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International Journal of Radiation Biology Volume 58, 1990 - Issue 5
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Technical Note

An in Vitro Clonogenic Assay to Assess Radiation Damage in Rat CNS Glial Progenitor Cells

R.W.M. van der Maazen Institute of Radiotherapy, University of Nijmegen, 6500 HB, Nijmegen, The Netherlands
,
I. Verhagen Institute of Radiotherapy, University of Nijmegen, 6500 HB, Nijmegen, The Netherlands
&
A.J. van der Kogel Institute of Radiotherapy, University of Nijmegen, 6500 HB, Nijmegen, The Netherlands
Pages 835-844 | Received 04 Mar 1990, Accepted 06 May 1990, Published online: 03 Jul 2009
 
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Summary

Normal glial progenitor cells can be isolated from the rat central nervous system (CNS) and cultured in vitro on a monolayer of type-1 astrocytes. These monolayers are able to support and stimulate explanted glial progenitor cells to proliferate. Employing these in vitro interactions of specific glial cell types, an in vivo-in vitro clonogenic assay has been developed. This method offers the possibility to study the intrinsic radiosensitivity, repair and regeneration of glial progenitor cells after in vitro or in vivo irradiation.

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