Dependence of the Yield of Strand Breaks Induced by γ-rays in DNA on the Physical Conditions of Exposure: Water Content and Temperature

Abstract

The induction by ⁶⁰Co γ-rays of DNA breaks, revealed by relaxation (single-strand breaks, SSBs) and linearization (double-strand breaks, DSBs) of supercoiled plasmid DNA, was measured under three irradiation conditions, the DNA being in a dry, humid, or aqueous state in the absence of oxygen, at 25 or −196°C (77K). Yields of strand breaks (3·0 × 10⁻¹⁰ SSB/Gy · Da and 2·6 × 10⁻¹¹ DSB/Gy · Da) in DNA exposed to a stream of humidified nitrogen were higher than those in the dry condition (5·7 × 10⁻¹¹ SSB/Gy · Da and 3·2 × 10⁻¹² DSB/Gy · Da), but both these yields were markedly lower than those measured for DNA in aqueous solution at a concentration of 73 µg/cm³ (1·14 × 10⁻⁷ SSB/Gy · Da and 5·4 × 10⁻⁹ DSB/Gy · Da). Over 100-fold fewer SSBs were observed in the frozen aqueous system compared with the non-frozen liquid state, whereas in the dry and humid states, freezing did not affect the yield as much. The same trend was observed for DSBs. However, the induction of SSBs was more affected than that of DSBs by freezing in the aqueous systems. An interesting reverse relationship was observed in humid systems. The observed linearity of DSB induction with radiation dose supported a single-event mechanism. A comparison of G values for humid systems revealed that the role of bound water in radiation damage becomes significant in the nonfrozen state. Based on these and other measurements of strand breaks under different conditions, the significance of bound and free water on the yields of DNA strand breaks by γ-rays is discussed, and the relevance of these results to the in vivo situation outlined.