An Ionizing Radiation-sensitive Mutant of CHO Cells: Irs-20. III. Chromosome Aberrations, DNA Breaks and Mitotic Delay

Abstract

The ionizing radiation-sensitive mutant of CHO cells, irs-20, showed a defect in the rate and extent of rejoining of γ-ray-induced DNA double-strand breaks (dsbs) compared with the parental CHO cells as measured using pulsed-field gel electrophoresis. Virtually all rejoined in wild-type cells but some 20–30% failed to rejoin in the mutant cells during a 5-h period after irradiation. An increased level of chromosome-type aberrations per unit dose was seen in irs-20 cells compared with wild-type cells irradiated during the G₁ phase. For the irs-20 cells, about half the dose was required to produce the same chromosome-type aberration frequency. Chromatid-type aberrations were induced in G₁-irradiated irs-20 cells at frequencies nearly the same as for chromosome types. For the parental wild-type CHO 10B2 cells, only chromosome types were seen. The distribution of aberrations among cells was not significantly different from Poisson for wild-type cells, but this was not the case for irs-20 cells where the overdispersion was highly significant. The mutant irs-20 cells displayed a much greater cell cycle delay per unit dose (about five-fold) in reaching mitosis after irradiation in G₁ than the more radioresistant wild-type parental cells.