Combined FISH Painting and Harlequin Staining for Cell Cycle-controlled Chromosome Analysis in Human Lymphocytes

U. Kulka GSF-Forschungszentrum für Umwelt und Gesundheit Neuherberg, Institut für Strahlenbiologie, Postfach 1129, D-85758, Oberschleißheim, Germany

R. Huber GSF-Forschungszentrum für Umwelt und Gesundheit Neuherberg, Institut für Strahlenbiologie, Postfach 1129, D-85758, Oberschleißheim, Germany

P. Müller GSF-Forschungszentrum für Umwelt und Gesundheit Neuherberg, Institut für Strahlenbiologie, Postfach 1129, D-85758, Oberschleißheim, Germany

S. Knehr GSF-Forschungszentrum für Umwelt und Gesundheit Neuherberg, Institut für Strahlenbiologie, Postfach 1129, D-85758, Oberschleißheim, Germany

M. Bauchinger GSF-Forschungszentrum für Umwelt und Gesundheit Neuherberg, Institut für Strahlenbiologie, Postfach 1129, D-85758, Oberschleißheim, Germany

Abstract

We present a simple method that allows scoring of FISH-painted chromosomes exclusively in the first division human lymphocytes. It consists of a combination of FISH with chromosome-specific libraries and a differential sister chromatid staining after BrdU treatment of lymphocyte cultures. The method allows a precise quantification of induced chromosome damage for human biodosimetry.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Combined FISH Painting and Harlequin Staining for Cell Cycle-controlled Chromosome Analysis in Human Lymphocytes

Information for

Open access

Opportunities

Help and information

Combined FISH Painting and Harlequin Staining for Cell Cycle-controlled Chromosome Analysis in Human Lymphocytes

Abstract

Reprints and Corporate Permissions

Academic Permissions

Related research

To cite this article:

Download citation

Information for

Open access

Opportunities

Help and information

Keep up to date

Your download is now in progress and you may close this window

Login or register to access this feature