DNA damage induction and tumour cell radiosensitivity: PFGE and halo measurements

Abstract

Purpose: To test whether induction of DNA damage is correlated with tumour-cell radiosensitivity. Materials and methods: Initial DNA damage caused by X-irradiation was measured in ten human tumour cell lines, which largely differed in radiosensitivity, using either the pulsed-field gel electrophoresis assay or the halo technique. Results: None of the parameters of DNA damage correlated with any parameter of cellular radiosensitivity. This was not only true when the analysis was performed on all data but also when the analysis was performed after separating the cell lines into radioresistant and sensitive groups. Even when differences in chromosome number, ploidy and cell cycle distribution were taken into account, no significant correlations were obtained. Conclusions: Contrary to previous suggestions, differences in the number of double-strand breaks induced or chromatin-related 'presentation' of DNA lesions, measured by pulsed-field gel electrophoresis or halo respectively, are generally not the dominant factors determining tumour-cell radiosensitivity.